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Continuous production of maltotetraose using immobilized Pseudomonas stutzeri amylase
Author(s) -
Kimura Takashi,
Ogata Masafumi,
Yoshida Masahiro,
Nakakuki Teruo
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320512
Subject(s) - pseudomonas stutzeri , chemistry , hydrolysis , industrial and production engineering , yield (engineering) , substrate (aquarium) , amylase , chromatography , adsorption , immobilized enzyme , bioreactor , enzyme , materials science , organic chemistry , bacteria , biology , ecology , genetics , electrical engineering , metallurgy , engineering
A continuous production process of maltotetraose was investigated by using immobilized maltotetraose (G 4 )‐ forming amylase (1,4‐α‐ D ‐glucan maltotetraohydrolase, EC3.2.1.60) from Pseudomonas stutzeri adsorbed on a macroporous hydrophobic resin. The maximum reaction rate was obtained at 55°C and the activation energy of hydrolysis by immobilized G 4 ‐forming amylase was calculated to be 8.45 kcal/mol. The maltotetraose yield was greatly influenced by the flow rate of substrate solution, its concentration, and the immobilized enzyme activity. The newly defined factor “specific space velocity” was successfully introduced to normalize the operating parameters. Using this factor, the immobilized enzyme reactor then can be simulated and the operating dynamics can be determined.