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Properties of invertase immobilized on the poly(ethylene‐co‐vinyl alcohol) hollow fiber membrane
Author(s) -
Shiomi Tomoo,
Tohyama Masao,
Satoh Mikitoshi,
Miya Masamitsu,
Imai Kiyokazu
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320511
Subject(s) - invertase , vinyl alcohol , chemistry , immobilized enzyme , sucrose , fiber , ethylene , membrane , yield (engineering) , alcohol , chromatography , substrate (aquarium) , michaelis–menten kinetics , enzyme , enzyme assay , nuclear chemistry , polymer chemistry , organic chemistry , biochemistry , catalysis , materials science , polymer , composite material , oceanography , geology
Invertase was ionically immobilized on the poly(ethylene‐co‐vinyl alcohol) hollow fiber inside surface, which was aminoacetalized with 2‐dimethylaminoacetaldehyde dimethyl acetal. Immobilization and enzyme reaction were carried out by letting the respective solutions pass or circulate through the inside of the hollow fiber, and the activity of invertase was determined by the amount of glucose produced enzymatically from sucrose. Immobilization conditions were examined with respect to the enzyme concentration and to the time, and consequently the preferable conditions at room temperature were found to be 5 μg/mL of enzyme concentration and 4 h of immobilization time. Under those conditions the immobilization yield and the ratio of the activity of the immobilized invertase to that of the native one were 89 and 80%, respectively. For both repeating and continuous usages, the activity fell to ca. 60% of the initial activity in the early stage and after that almost kept that value. The apparent Michaelis constant K m ′for the immobilized invertase decreased with increasing the flow rate of the substrate solution, to be close to the value for the native one. Furthermore, the possibility of the separation of the enzymatically formed glucose from the reaction mixture through the hollow fiber membrane was preliminarily examined.