Premium
Mathematical modeling of affinity ultrafiltration process
Author(s) -
Luong J. H. T.,
Male K. B.,
Nguyen A. L.,
Mulchandani A.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320407
Subject(s) - ultrafiltration (renal) , trypsin , chemistry , chromatography , filtration (mathematics) , yield (engineering) , process (computing) , chymotrypsin , enzyme , computer science , materials science , biochemistry , mathematics , statistics , metallurgy , operating system
An affinity ultrafiltration process has been developed by exploiting affinity binding in conjunction with cross‐flow filtration. The process was proven to possess high resolution, high recovery yield, and ease of scale‐up. The process could purify trypsin from a trypsin‐chymotrypsin mixture batchwise or continuously. Essential for applying this concept was the synthesis of a water‐soluble high‐molecular‐weight polymer bearing m ‐aminobenzamidine, a strong and specific trypsin in hibitor. A mathematical model was also developed to describe the dynamic behavior of the newly developed purification process. The model was able to predict the profiles of enzyme concentrations in the process with high accuracy.