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Immobilization of glucose oxidase on polymer membranes treated by low‐temperature plasma
Author(s) -
Kawakami Mitsuyasu,
Koya Hidekazu,
Gondo Shinichiro
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320316
Subject(s) - polyvinylidene fluoride , membrane , glutaraldehyde , glucose oxidase , immobilized enzyme , chemistry , polymer , biosensor , chromatography , fluoride , polytetrafluoroethylene , polymer chemistry , chemical engineering , enzyme , biochemistry , organic chemistry , inorganic chemistry , engineering
Low‐temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a linking agent. Enzyme activity was evaluated by the response of glucose sensor composed of the immobilized enzyme membrane and a dissolved oxygen electrode. The sensor response was found to depend on the kind of carrier membrane and to become maximum at suitable conditions of plasma treatment.