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Controlled fed‐batch fermentation of recombinant Saccharomyces cerevisiae to produce hepatitis B surface antigen
Author(s) -
Hsieh JihHan,
Shih KunYu,
Kung HsiangFu,
Shiang Ming,
Lee LanYing,
Meng MengHsiao,
Chang ChingChuan,
Lin HuiMin,
Shih ShuChing,
Lee ShuYing,
Chow TehYuan,
Feng TengYung,
Kuo TsongTeh,
Choo KongBung
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320310
Subject(s) - hbsag , fermentation , yeast , saccharomyces cerevisiae , dehydrogenase , alcohol dehydrogenase , biology , biochemistry , antigen , strain (injury) , acid phosphatase , chemistry , microbiology and biotechnology , enzyme , virology , hepatitis b virus , immunology , virus , anatomy
We have performed controlled fed‐batch fermentation experiments to compare the production level of hepatitis B surface antigen (HBsAg) by recombinant yeast Saccharomyces cerevisiae strains (YNN27/pYBH‐1, YNN27/ p2μ‐S11, YNN27/pDCB‐S2) containing plasmid vector with alcohol dehydrogenase (ADH1), acid phosphatase (PHO5), and glyceraldehyde‐3‐phosphate dehydrogenase (GPD) promoter, respectively. Yeast cell concentrations of 15‐35 g dry cell weight/L were obtained. By limiting phosphorous concentration, HBsAg expression level for the YNN27/p2μ‐S11 strain with inducible PHO5 promoter reached 0.2–0.3 mg/L. By controlling nutrient addition rate and dissolved oxygen concentration, HBsAg concentrations of 3‐10 mg/L were achieved in 60–70 h fermentation using the YNN27/pDCB‐S2 strain with the constitutive GPD promoter.