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Fluorescence sensing of fermentation parameters using fiber optics
Author(s) -
Junker B. H.,
Wang D. I. C.,
Hatton T. A.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260320109
Subject(s) - fluorometer , fermentation , fluorescence , yeast , optical fiber , fiber , optical density , chromatography , chemistry , industrial fermentation , analytical chemistry (journal) , biological system , optics , biology , biochemistry , physics , organic chemistry
A method has been developed for measuring fermentation parameters such as dissolved oxygen, pH, and cell density that differs from traditional techniques that require electrodes and off‐line samples. Fluorescent indicators, each sensitive to a single variable, are dissolved directly into a fermentation broth. A fiber‐optic probe fluorimeter measures the fluorescence intensities that can then be correlated with parameter values. In addition, an integrated scatter scanning technique can be used to monitor cell density in situ. Results have been obtained using simulated baker's yeast broth and during actual baker's yeast fermentations.