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Simultaneous measurements of oxygen diffusion coefficients and solubilities in fermentation media with polarographic oxygen electrodes
Author(s) -
Ju LuKwang,
Ho Chester S.,
Baddour Raymond F.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310912
Subject(s) - fermentation , diffusion , oxygen , chemistry , clark electrode , penicillium chrysogenum , polarography , limiting oxygen concentration , solubility , chromatography , analytical chemistry (journal) , inorganic chemistry , electrode , biochemistry , thermodynamics , organic chemistry , physics , electrolyte
A membrane‐covered oxygen electrode was used to measure oxygen diffusion coefficients and solubilities in aqueous glucose solutions and various fermentation media following a newly developed methodology. The fermentation media studied were tryptic soy broth and those for fermentations of Penicillium chrysogenum, Saccharomyces cerevisiae , and Micrococcus glutamicus . The experimental results of oxygen diffusion coefficients and solubilities in glucose solutions were in good accord with the literature data. As for the fermentation media, both oxygen diffusion coefficients and solubilities were found to decrease with an increased fractional composition of these media, and log‐additive behaviors of the oxygen diffusion coefficients and solubilities in fermentation media were observed.