Premium
Continuous fractionation of human plasma proteins by precipitation from the suspension of the recycling stream
Author(s) -
Chang Chong E.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310812
Subject(s) - fractionation , suspension (topology) , precipitation , chromatography , chemistry , human plasma , suspension culture , environmental chemistry , environmental science , biology , cell culture , meteorology , homotopy , pure mathematics , genetics , physics , mathematics
The conventional cold‐ethanol batch fractionation method of human plasma is converted to an automatically controlled continuous fractionation process. The selected protein fractions are precipitated by mixing in the recycled product stream of the suspension. Compared to the batch process, the continuous fractionation process generates less coprecipitation and less spontaneous nucleation, allowing efficient centrifugation of precipitates, and the yield and purity of albumin in the final fraction is significantly increased.