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Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde
Author(s) -
Duff Sheldon J. B.,
Murray William D.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310805
Subject(s) - acetaldehyde , pichia pastoris , ethanol , chemistry , chromatography , biochemistry , recombinant dna , gene
Free and immobilized cells of Pichia pastoris were used to convert ethanol to acetaldehyde in small‐scale batch reactors. Immobilized cells were less active than free cells ( V max free = 7.81 g/L h, V max immobilized = 3.17 g/L h) due to a number of factors including end product inhibition and diffusional limitations. Immobilized cells were more resistant to heat denaturation both in the presence and absence of ethanol. Immobilized cells retained more of their activity during repeated batch cycles than did free cells.