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Two‐Liquid phase reactor studies of 11α‐hydroxylation of progesterone by Aspergillus ochraceus
Author(s) -
Ceen E. G.,
Dunnili P.,
Herrmann J. P. R.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310718
Subject(s) - aspergillus ochraceus , hydroxylation , chemistry , chromatography , aqueous solution , aqueous two phase system , phase (matter) , ochratoxin a , organic chemistry , mycotoxin , food science , enzyme
Abstract The 11α‐hydroxylation of progesterone by free cells of Aspergillus ochraceus has been examined in a reactor with an aqueous and a natural oil phase. As the proportion of oil is increased, the point of inversion from a continuous aqueous phase is affected by the cell concentration, as is the optimum ratio of the phases for conversion. High oil ratios are not productive because of poor mass transfer to the concentrated cells in the aqueous phase. The cells are stable in the presence of oleic acid for at least 24 h.