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Characterization of fractionated polyclonal antibodies for immunoaffinity chromatography
Author(s) -
Sada Eizo,
Katoh Shigeo,
Kiyokawa Atsuo,
Kondo Akihiko
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310702
Subject(s) - chemistry , ionic strength , chromatography , polyclonal antibodies , affinity chromatography , elution , fractionation , dissociation constant , affinities , dissociation (chemistry) , ionic bonding , antibody , biochemistry , aqueous solution , enzyme , organic chemistry , ion , biology , receptor , immunology
Polyclonal anti‐BSA antibodies were ractionated by stepwise elution from an immobilized BSA column by decreasing pH or increasing the concentration of NaSCN. The binding affinities of each fraction and original globulin under physiological conditions and their dependence on pH and ionic environments were compared. Fractions with high association constant under physiological conditions did not necessarily show antigen binding affinity over a wide pH range, but they retained a high affinity at higher ionic strength of NaSCN. Consequently, by combining these two fractionation procedures, a fraction with high affinity and which dissociated at moderate pH was obtained. It is clear that high affinity is not always incompatible with ease of dissociation accompanying a change in conditions.