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Gluconic acid production by an mobilized glucose oxidase reactor with electrochemical regeneration of an artificial electron acceptor
Author(s) -
Bourdillon Christian,
Lortie Robert,
Laval Jean Marc
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310607
Subject(s) - gluconic acid , glucose oxidase , electron acceptor , chemistry , regeneration (biology) , electrochemistry , oxidase test , acceptor , biochemistry , chemical engineering , combinatorial chemistry , enzyme , microbiology and biotechnology , biology , electrode , physics , condensed matter physics , engineering
Abstract A reactor, using the enzymatic electrocatalysis scheme, was developed on a laboratory preparative scale for the catalytic oxidation of glucose into gluconic acid. Glucose oxidase was directly immobilized on the surface of a carbon felt electrode and a solution of glucose and benzo‐quinone passed through the electrode in order to regenerate the electron acceptor. The reactor was able to produce continuously 1.5 g gluconate/h with a catalytic current of 0.4 A. This gave a high productivity ca. 100 g/h/L reactor. A one‐dimensional model was developed which demonstrated the efficiency of coupling between enzymatic and electrochemical reactions due to the proximity of the two reaction sites. For example the catalytic current was practically independent of mass transfer parameters. The operational stability of immobilized glucose oxidase was increased 50 times at least when electroregenerated benzoquinone was used as oxidant instead of oxygen.