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Effect of cycling on the stability of plasmid‐bearing microorganisms in continuous culture
Author(s) -
Stephens M. L.,
Lyberatos G.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310511
Subject(s) - cycling , adaptability , plasmid , strain (injury) , substrate (aquarium) , bearing (navigation) , temperature cycling , microorganism , biological system , stability (learning theory) , biochemical engineering , biology , computer science , ecology , engineering , thermodynamics , bacteria , biochemistry , genetics , physics , dna , history , archaeology , anatomy , artificial intelligence , thermal , machine learning
Abstract The implication of the possible existence of differences in the times required for plasmid‐bearing and non‐plasmid‐bearing microorganisms to adjust their metabolic activities to step changes in their environment is examined. This adaptability difference suggests the possibility of maintaining an engineered strain in continuous culture by transient operation. It is shown for the case where adaptability is neglected that no cycling strategy will prevent the washout of the engineered strain, but the addition to the model of a time delay in substrate utilization can result in coexistence upon cycling. Numerical simulations of cycling in feed substrate concentration are carried out to illustrate the concept Operating diagrams are also constructed to indicate the conditions under which washout of the plasmid bearing strain can be prevented.