Premium
Production of optically active 2,3‐butanediol by Bacillus polymyxa
Author(s) -
De Mas Carles,
Jansen Norman B.,
Tsao George T.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310413
Subject(s) - acetoin , 2,3 butanediol , yield (engineering) , paenibacillus polymyxa , butanediol , oxygen , fermentation , chemistry , ethanol , metabolite , biochemistry , organic chemistry , bacteria , biology , materials science , metallurgy , genetics
Bacillus polymyxa produces ( R , R )‐2,3‐butanediol from a variety of carbohydrates. Other metabolites are also produced including acetoin, acetate, lactate, and ethanol. The excretion of each metabolite was found to depend on the relative availability of oxygen to the culture. When the relative oxygen uptake rate was high, enhanced yields of acetate and acetoin were noted. At an intermediate oxygen availability, the butanediol yield was maximal. When the availability of oxygen was more restricted, higher yields of lactate and ethanol occurred. The cells appeared to regulate themselves such that energy generation is optimal subject to the constraint that the cells do not produce more reducing equivalents than can be oxidized by the electron transport system. The dependence of each product yield on the relative oxygen availability was determined, and this knowledge was used to carry out a fed‐batch fermentation that attained a final butanediol concentration of over 40 g/L in 50 h.