Premium
Kinetics of α‐amylase synthesis from Bacillus amyloliquefaciens
Author(s) -
Yoo Young J.,
Cadman Theodore W.,
Hong Juan,
Hatch Randolph T.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310412
Subject(s) - maltose , bacillus amyloliquefaciens , catabolite repression , amylase , chemistry , biochemistry , substrate (aquarium) , alpha amylase , fermentation , food science , enzyme , biology , ecology , mutant , gene
The microbial production of α‐amylase from Bacillus amyloliquefaciens was investigated. The microorganism was grown using media containing glucose or maltose at 37°C and under aerobic conditions in a 16‐L fermentor. The α‐amylase synthesis from maltose was not found to be inducible but was found to be subject to catabolite repression. The maltose uptake rate was observed to be the rate‐limiting step compared to the conversion rate of maltose to glucose by intracellular α‐glucosidase. The α‐amylase activity achieved with maltose as a substrate was higher than that achieved with glucose. A slower growth rate and a higher cell density were obtained with maltose. The enzyme production pattern depended upon the nutrient composition of the medium.