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High‐Density culture of mouse–human hybridoma in serum‐free defined medium
Author(s) -
Takazawa Yoshiharu,
Tokashiki Michiyuki,
Murakami Hiroki,
Yamada Koji,
Omura Hirohisa
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310210
Subject(s) - transferrin , monoclonal antibody , chemically defined medium , chemistry , cell culture , antibody , microbiology and biotechnology , biology , biochemistry , immunology , genetics , in vitro
Mouse–human hybridoma 4H11 cells producing anti‐ Pseudomonas sp. monoclonal antibody (IgA) grew in a serum‐free medium supplemented with insulin, transferrin, ethanolamine, and selenite (ITES). The hybridoma could be applied to high‐density culture in a serum‐free medium supplemented with ITES, 0.5% BSA, egg yolk VLDL, and artificial blood FC‐43 in a culture vessel equipped with hollow‐fiber modules for medium exchange. Total cell density reached 1.1 × 10 7 cells/mL (viable cell density was 7.6 × 10 6 cells/mL), and the IgA productivity was around 20 μg/10 6 cells/day in the serum‐free medium, which corresponded to the levels in serum‐supplemented medium.