Premium
In situ extractive fermentation of acetone and butanol
Author(s) -
Roffler S. R.,
Blanch H. W.,
Wilke C. R.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310207
Subject(s) - clostridium acetobutylicum , butanol , fermentation , acetone , oleyl alcohol , chromatography , extraction (chemistry) , chemistry , product inhibition , clostridium , ethanol , biochemistry , alcohol , biology , bacteria , enzyme , genetics , non competitive inhibition
The productivity of the acetone–butanol fermentation was increased by continuously removing acetone and butanol from the fermentation broth during fed‐batch culture. Whole broth containing viable cells of Clostridium acetobutylicum was cycled to a Karr reciprocating plate extraction column in which acetone and butanol were extracted into oleyl alcohol flowing counter‐currently through the column. By continuously removing these toxic metabolites from the broth, end product inhibition was reduced, and a concentrated feed solution containing 300 g/L glucose was fermented at an overall butanol productivity of 1.0 g/L h, 70% higher than the productivity of normal batch fermentation. The continuous extraction process provides flexible operation and lends itself to process scale‐up.