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Kinetics and regulation of β‐lactamase formation in methylotrophic bacterium
Author(s) -
Samuelov Nissim S.
Publication year - 1988
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260310205
Subject(s) - chemostat , enzyme , kinetics , strain (injury) , bacteria , bacterial growth , exponential growth , biochemistry , pseudomonas , chemistry , growth medium , growth rate , methanol , pseudomonadales , cell growth , biology , microbiology and biotechnology , organic chemistry , mathematical analysis , genetics , physics , mathematics , geometry , anatomy , quantum mechanics
The regulation and kinetics of formation of an inducible, cell‐bound oxacillin‐hydrolyzing β‐lactamase (M‐OXA) by a methanol‐grown Pseudomonas strain were investigated in batch, chemostat, and two‐stage continuous cultures. The extent to which enzyme production occurs declines at increased growth rates, and the rate of M‐OXA β‐lactamase production follows a kinetic pattern that is partially growth independent and partially inversely growth linked. Growth and enzyme formation are regulated differently by medium constituents. The initial steps of M‐OXA β‐lactamase synthesis takes place during the exponential growth phase, and active enzyme is produced by induced nonproliferating cells, probably through a turnover mechanism.