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Continuous cellulase production by immobilized Sporotrichum cellulophilum and continuous saccharification of bagasse
Author(s) -
Tamada Masao,
Kasai Noboru,
Kaetsu Isao
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300602
Subject(s) - bagasse , cellulase , hydrolysis , chemistry , pulp and paper industry , fermentation , slurry , dilution , chromatography , bioreactor , filter paper , food science , biochemistry , environmental science , organic chemistry , environmental engineering , engineering , physics , thermodynamics
Immobilized Sporotrichum cellulophilum with nonwoven materials was cultured continuously by a rotating‐disk fermentor to supply cellulase into the saccharification system. The filter paper activity (5.0) was retained after 696 h under conditions of 250 rpm stirring and 0.014 h −1 dilution rate. The product of the culture was supplied continuously to the saccharification reactor and used for the saccharification of bagasse. A glucose solution of ca. 0.9% was obtained continuously from 5% bagasse slurry during 610 h saccharification by this method.