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Ionic binding of biologically active proteins on cross‐linked acrylic macromolecular supports
Author(s) -
Maxim Steliana,
Flondor Aurelia,
Carpov Adrian
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300502
Subject(s) - chemistry , macromolecule , diffusion , ionic bonding , chromatography , biological activity , swelling , process (computing) , enzyme , polymer chemistry , biophysics , chemical engineering , biochemistry , organic chemistry , ion , in vitro , biology , physics , computer science , engineering , thermodynamics , operating system
The immobilization process of some biologically active proteins on acrylic ion exchange supports was studied. The cross‐linking and swelling coefficient, as well as the molecular weight of the enzyme, were proved to influence the immobilization process. The kinetic data indicate mainly a binding process controlled by diffusion. The uptake coefficient values of different proteins ranged between 35.0 and 98.0%. The laboratory experiments per formed with some of the obtained enzymic preparation certify the preservation of the catalytic activity following the immobilization process.