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Enzyme‐Entrapping behaviors in alginate fibers and their papers
Author(s) -
Kobayashi Yoshinari,
Matsuo Ryûkichi,
Ohya Takaichi,
Yokoi Nobumasa
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300316
Subject(s) - chemistry , calcium alginate , spinning , aqueous solution , polymer , entrapment , divalent , metal ions in aqueous solution , immobilized enzyme , pectinase , chelation , enzyme , chemical engineering , fiber , metal , polymer chemistry , chromatography , organic chemistry , calcium , medicine , surgery , engineering
Enzyme immobilization in the form of fiber and paper was easily achieved by wet spinning of aqueous admixture of sodium alginate and enzymes into divalent metallic ion solution as a coagulating bath, followed by paper making of resultant shortly cut fibers. Entrapment yields of enzymes used, e.g., glucoamylase, cyclodextrin glucanotransferase, endo‐polygalacturonase, and protease, were always higher in calcium alginate fibers and their papers than those in corresponding beads. It was found that the yields increased with an increase of the discharge rate through the spinning nozzle because the higher discharge rate could provide more highly oriented metal‐chelate linear polymer molecules along the fiber axis for preventing leakage of entrapped enzymes. Divalent metallic ions affected greatly the entrapment of glucoamylase in alginate fibers, the order of which followed roughly the ionotropic series of Thiele. Entrapment of glucoamylase in bicomponent systems comprising alginate and other water‐soluble polymers was also investigated.