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Immobilization of microorganisms by adhesion: Interplay of electrostatic and nonelectrostatic interactions
Author(s) -
Mozes N.,
Marchal F.,
Hermesse M. P.,
Van Haecht J. L.,
Reuliaux L.,
Leonard A. J.,
Rouxhet P. G.
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300315
Subject(s) - adhesion , zeta potential , isoelectric point , flocculation , chemistry , surface charge , x ray photoelectron spectroscopy , chemical engineering , contact angle , microorganism , materials science , nanotechnology , organic chemistry , nanoparticle , bacteria , biology , engineering , genetics , enzyme
The adhesion of three microorganisms ( Saccharomyces cerevisiae, Acetobacter aceti , and Moniliella pollinis ) to different materials has been studied using various supports (glass, metals, plastics), some of which were treated by an Fe(III) solution. The surface properties of the cells were characterized by the zeta potential and an index of hydrophobicity; characterization of the supports involved surface chemical analysis (XPS) and contact angle measurements. Cell suspensions in pure water at a given pH were left to settle on plates; the latter were then rinsed and examined microscopically, Saccharomyces cerevisiae and A. aceti adhere to metals under certain pH conditions but do not adhere to any of the other materials tested unless it is previously treated by ferric ions; adhesion of these hydrophilic cells is essentially controlled by electrostatic interactions. Moniliella pollinis adhere spontaneously to glass and to polymeric materials, but its attachment is also influenced by cell–cell or cell–support electrostatic repulsions; near the cell isoelectric point, cell flocculation is competing with adhesion to a support.