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Lipase reaction in AOT‐isooctane reversed micelles: Effect of water on equilibria
Author(s) -
Han Daeseok,
Rhee Joon Shick,
Lee Sun Bok
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300309
Subject(s) - chemistry , micelle , reaction rate , reaction rate constant , hydrolysis , lipase , aqueous solution , substrate (aquarium) , thermodynamics , equilibrium constant , pulmonary surfactant , michaelis–menten kinetics , chromatography , kinetics , organic chemistry , catalysis , enzyme , enzyme assay , biochemistry , physics , oceanography , quantum mechanics , geology
The effect of water on equilibria for hydrolytic reaction in reversed micelles has been investigated using lipase as a model enzyme. The effect of water on equilibria has been ignored for hydrolase reactions in an aqueous phase. In a reversed micellar system, however, the equilibrium of the lipase reaction was changed when water was added during the hydrolytic reaction. Furthermore, equilibrium fractional conversion is affected by the initial water concentration, being shifted to higher values with higher water concentrations, with other reaction conditions being held constant, indicating that the reaction should be regarded as a two‐substrate process. Equations corresponding to a two‐substrate, second‐order reversible model are derived and used for further analysis. The progress curves predicted from the rate equations agree very well with the experimental results under various reaction conditions. The values of the molar ratio of water to surfactant ( R ) which maximize the initial reaction rate and maximum fractional conversion is predictable from the derived rate equations and the resulting relationship between R and the kinetic constants.