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Effect of activation energy microheterogeneity on first‐order enzyme deactivation
Author(s) -
Malhotra Arun,
Sadana Ajit
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300116
Subject(s) - enzyme , kinetics , chemistry , activation energy , enzyme assay , thermodynamics , biophysics , biochemistry , physics , biology , classical mechanics
The influence of microheterogeneity on enzyme inactivation kinetics is examined. A continuous normal distribution of the thermal activation energy is assumed, and using this, a simple mathematical model is developed to find the activity–time trajectories for a microheterogeneous enzyme. Using an example, the model is used to show the quantitative effects of microheterogeneity such as increased order and stability observed during an enzyme inactivation. Experimental measurement of the extent of microheterogeneity in an enzyme sample is also discussed.