Premium
Rules for optimization of biocatalysis in organic solvents
Author(s) -
Laane Colja,
Boeren Sjef,
Vos Kees,
Veeger Cees
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260300112
Subject(s) - biocatalysis , partition coefficient , chemistry , solvent , organic solvent , substrate (aquarium) , stereochemistry , organic chemistry , reaction mechanism , catalysis , chemical engineering , biology , engineering , ecology
General rules for the optimization of different biocatalytic systems in various types of media containing organic solvents are derived by combining data from the literature, and the logarithm of the partition coefficient, log P , as a quantitative measure of solvent polarity. (1) Biocatalysis in organic solvents is low in polar solvents having a log P < 2, is moderate in solvents having a log P between 2 and 4, and is high in a polar solvents having a log P > 4. It was found that this correlation between polarity and activity parallels the ability of organic solvents to distort the essential water layer that stabilizes the biocatalysts. (2) Further optimization of biocatalysis in organic solvents is achieved when the polarity of the microenvironment of the biocatalyst (log P i ) and the continuous organic phase (log P cph ) is tuned to the polarities of both the substrate (log P s ) and the product (log P p ) according to the following rules: |log P i − log P s | and |log P cph − log P p | should be minimal and |log P cph − log P s | and |log P i − log P p | should be maximal, with the exception that in the case of substrate inhibition log P i , should be optimized with respect to log P s In addition to these simple optimization rules, the future developments of biocatalysis in organic solvents are discussed.