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Isolation of a respiratory‐deficient Kluyveromyces fragilis mutant for the production of ethanol from Jerusalem artichoke
Author(s) -
Guiraud Joseph P.,
Bourgi Jamal,
Stervinou Michèle,
Claisse Maurice,
Galzy Pierre
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260290707
Subject(s) - jerusalem artichoke , mutant , inulinase , ethidium bromide , mutagenesis , chemistry , ethanol , isolation (microbiology) , yeast , biology , microbiology and biotechnology , biochemistry , fermentation , gene , dna
A respiratory‐deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a 3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especially from Jerusalem artichoke extracts containing large amounts of high polyfructosans ("early" extracts).