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A new method to determine active enzyme distribution, effective diffusivity, rate constant for main reaction and rate constant for deactivation
Author(s) -
Do D. D.,
Hossain Md. M.
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260290502
Subject(s) - thermal diffusivity , reaction rate constant , constant (computer programming) , chemistry , hydrogen peroxide , substrate (aquarium) , thermodynamics , reaction rate , mass diffusivity , porosity , kinetics , organic chemistry , catalysis , classical mechanics , physics , oceanography , computer science , programming language , geology
A new method is presented to determine (1) the rate constant for the main reaction, (2) the rate constant for deactivation, (3) the effective diffusivity, and (4) the active enzyme distribution within a porous solid support by utilizing data of bulk substrate concentration versus time in a continuous stirred basket reactor. The method relies on an assumption of parallel deactivation mechanism with strong pore diffusional resistance with respect to substrate species. The data of hydrogen peroxide‐immobilized catalase published in the literature are used to demonstrate the theory. A parameter determination procedure is also presented.