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A model for growth of Saccharomyces cerevisiae containing a recombinant plasmid in selective media
Author(s) -
Sardonini Charles A.,
DiBiasio David
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260290410
Subject(s) - plasmid , saccharomyces cerevisiae , recombinant dna , metabolite , strain (injury) , auxotrophy , biology , cell growth , population , microbiology and biotechnology , chemistry , yeast , escherichia coli , biochemistry , gene , demography , sociology , anatomy
A major problem in the use of plasmids as recombinant vectors is the problem of plasmid‐free cell generation from plasmid shedding and subsequent growth. A common technique for controlling the population of plasmidfree cells is the use of selective media against these cells using an auxotrophic host and a plasmid that has the ability to produced the essential metabolite. A distributed model describing the growth of Saccharomyces cerevisiae containing a recombinant plasmid in selective media was developed. The model allows for growth and production of a metabolite by the plasmid‐carrying strain and growth of the plasmid‐free cells on resulting metabolite concentrations. Through a determination of system constants and numerical solution to the equations, experimental batch and continuous culture results for cell concentration transients could be simulated by the model. The results indicated that despite selective pressure, plasmid‐free cell growth was significant.