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Adsorption of enzymes on krill chitin modified with carbon disulfide
Author(s) -
Synowiecki Józef,
SikorskaSiondalska Anna,
ElBedawey Abo ElFath
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260290309
Subject(s) - chitin , adsorption , chemistry , antarctic krill , enzyme , ionic strength , disulfide bond , carbon fibers , denaturation (fissile materials) , ionic bonding , organic chemistry , chemical engineering , krill , chromatography , biochemistry , chitosan , nuclear chemistry , materials science , biology , aqueous solution , composite number , ecology , ion , engineering , composite material
Krill chitin modified with carbon disulfide was used as a support of enzymes. The dithiocarbamino groups created after reaction of CS 2 with chitinous NH 2 can participate in binding the enzymes without denaturation and loss in activity. The stability of these bonds depends on pH and decreases gradually at ionic strengths higher than 0.01. Chitin modified with CS 2 can be used as a support for enzymes having high activity at mild conditions of pH and ionic strength.