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Fractionation of Aspergillus niger cellulases by combined ion exchange affinity chromatography
Author(s) -
Boyer R. F.,
Allen T. L.,
Dykema P. A.
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260290206
Subject(s) - fractionation , chemistry , chromatography , cellulase , ion chromatography , ion exchange , ion , organic chemistry , enzyme
Eight chemically modified cellulose supports were tested for their ability to absorb components of the Aspergillus niger cellulase system. At least two of the most effective adsorbents, aminoethyl cellulose and carboxymethyl cellulose, were shown to be useful for the fractionation of cellulases. These supports apparently owe their resolving capacity to both ion exchange and biospecific binding effects; however, the relative importance of each effect is unknown. These observations form the basis for a new cellulase fractionation technique, combined ion exchange–affinity chromatography.