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Cellulase immobilized on poly‐ L ‐glutamic acid
Author(s) -
Takeuchi Tamio,
Makino Keisuke
Publication year - 1987
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260290203
Subject(s) - cellulase , chemistry , solvolysis , cellulose , glutamic acid , hydrolysis , chromatography , nuclear chemistry , biochemistry , amino acid
Cellulase (Cellulosin AC‐8) was immobilized on poly‐ L ‐glutamic acid. This immobilized cellulase (IC) is water soluble in the neutral and alkaline solutions, where IC has the activity, while IC can be made insoluble by lowering the pH so that it can be recovered from the reaction mixture with its activity. The optimum pH and temperature were determined to be 5.5 and 55°C, respectively. The stability of IC against change in the pH and temperature was improved by the immobilization. Solvolysis of 3 N ‐NaOH‐treated cellulose, with IC under the optimum conditions found here, led to the production of low‐molecular‐weight compounds.