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The application of a novel heat flux calorimeter for studying growth of Escherichia coli W in aerobic batch culture
Author(s) -
Marison I. W.,
von Stockar U.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281205
Subject(s) - calorimeter (particle physics) , calorimetry , escherichia coli , fermentation , chemistry , heat flux , in situ , flux (metallurgy) , biological system , reaction calorimeter , metabolic flux analysis , thermodynamics , chromatography , biochemistry , biology , heat transfer , physics , metabolism , organic chemistry , optics , detector , gene
The modification and principle of a novel heat flux calorimeter for the in situ , on‐line measurement of the heat generated during microbial growth is described. Data concerning the physical characterization of the calorimeter as a fermentor, including stability and sensitivity of the heat signal, are presented. The calorimeter has been successfully applied to the study of the aerobic batch culture of Escherichia coli W on glucose under carbon and nitrogen limitation. A direct correlation between growth and heat evolution was obtained. Quantitative analysis of the data suggests that the new calorimetric technique could be used for monitoring growth and specific metabolic events, for convenient medium optimization, and as a basis for a novel fermentation process control system.