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Regeneration of NAD + cofactor by photosensitized electron transfer in an immobilized alcohol dehydrogenase system
Author(s) -
Julliard M.,
Le Petit J.,
Ritz P.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281204
Subject(s) - nad+ kinase , cofactor , chemistry , alcohol dehydrogenase , polyacrylamide , glycerol 3 phosphate dehydrogenase , oxidoreductase , ethanol , photosensitizer , electron transfer , immobilized enzyme , alcohol , enzyme , alcohol oxidoreductase , photochemistry , chromatography , organic chemistry , polymer chemistry
The irradiation with visible light of a photosensitizer dye like methylene blue was used to regenerate by electron transfer the oxidized form of a pyridine nucleotide coenzyme (NAD + ). The process has been studied on a common enzymatic reaction: ethanol oxidation by alcohol–NAD + oxidoreductase immobilized on polyacrylamide gel or porous glass balls. In the experimental conditions used, the initial NAD + recycling rates were 2.33 × 10 4 cycles/h (polyacrylamide) and 3 × 10 4 cycles/h (glass balls). A total number of 49.5 × 10 4 cycles was obtained for 13 runs of 2 h. The enzyme immobilization strongly increased its stability: after 28 days at 20°C, the residual activity was 25% of the initial value.