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In situ determination of kinetic parameters for biofilms: Isolation and characterization of oligotrophic biofilms
Author(s) -
Rittmann Bruce E.,
Crawford LouAnn,
Tuck Cynthia K.,
Namkung Eun
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281120
Subject(s) - biofilm , in situ , isolation (microbiology) , chemistry , biological system , biology , microbiology and biotechnology , bacteria , genetics , organic chemistry
This article develops and utilizes an in situ technique to estimate the Monod half‐maximum rate concentration, K s and the maximum specific utilization rate constant, k , for biofilms. The technique employs a curve‐matching method with kinetic results from several short‐term experiments with completely mixed biofilm reactors. Use of the in situ method eliminates the two drawbacks of using conventional suspended‐growth measurements to characterize biofilm: possible alteration of cell physiology and a major investment to run the suspended‐growth tests. Results with five cultures of biofilm‐forming oligotrophs demonstrated the in situ technique and supported the hypothesis that K s values were lower for the biofilm oligotrophs than for typical copiotrophs.