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Depolymerization of chondroitin C Sulfate by immobilized Proteus vulgaris cells
Author(s) -
Sato Nobuyuki,
Murata Kousaku,
Kimura Akira
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281114
Subject(s) - depolymerization , chemistry , chondroitin sulfate , enzyme , inducer , proteus vulgaris , incubation , biochemistry , enzyme assay , carrageenan , chondroitin , chromatography , nuclear chemistry , glycosaminoglycan , organic chemistry , escherichia coli , gene
Abstract Chondroitinase ABC catalyzing the depolymerization of chondroitin sulfate was induced by incubating the Proteus vulgaris cells in a medium containing chondroitin C sulfate as an inducer. Incubation of P. vulgaris cells for 12 h in the presence of 0.3% inducer was optimal to obtain the cells with highly active chondroitinase ABC. Such cells were immobilized in k‐carrageenan gel lattice, and some properties of chondroitinase ABC in immobilized cells were studied in comparison with those of the enzyme without immobilization (free enzyme). The stabilities of the enzyme toward heat and storage were remarkably improved by immobilizing the cells in k ‐carrageenan gel lattice. Optimal pH and temperature for activity of the enzyme were slightly shifted to the alkaline region and higher temperature by immobilization and were 9.0 and 35°C, respectively.