Premium
Kinetics and mechanism of dissimilative Fe(III) reduction by Pseudomonas sp. 200
Author(s) -
Arnold Robert G.,
Olson Terese M.,
Hoffmann Michael R.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281110
Subject(s) - nitrilotriacetic acid , ferrous , chemistry , kinetics , ferric iron , aqueous solution , genetic algorithm , ferric , siderophore , aqueous two phase system , nuclear chemistry , inorganic chemistry , pseudomonas , chelation , bacteria , biochemistry , organic chemistry , biology , physics , quantum mechanics , evolutionary biology , gene , genetics
The kinetics and mechanism of Fe(III) reduction to Fe(II) were studied in pure batch cultures of Pseudomonas sp. 200. The rate of iron reduction has been mechanistically related to aqueous phase iron speciation. In the absence of microbial activity the iron reduction rate was negligible. Initial rates of microbial iron reduction were accelerated more than 20‐fold by the addition of equimolar quantities of nitrilotriacetic acid (NTA) to media initially containing 1.86 × 10 −3 M total Fe(III). Numerical techniques were utilized to quantify relationships between the observed rate of Fe(II) production and the calculated (equilibrium) aqueous phase speciation. These results indicate that soluble ferric iron species are not equivalent in terms of their susceptibility to bacterial (dissimilative) iron reduction. The concentration of Fe(NTA)(OH) 2 2−correlated strongly with observed iron reduction rates. Ferrous iron species appeared to inhibit the reduction process.