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Growth kinetics of Dioscorea deltoidea and Catharanthus roseus in batch culture
Author(s) -
Drapeau D.,
Blanch H. W.,
Wilke C. R.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281014
Subject(s) - catharanthus roseus , sugar , diosgenin , respiration , fermentation , botany , respiration rate , fed batch culture , growth rate , chemistry , biology , food science , geometry , organic chemistry , mathematics
Sugar‐limited batch growth of Dioscorea deltoidea and Catharanthus roseus plant cell cultures was studied in a 14‐L stirred tank fermentor. With dissolved oxygen concentration monitored and maintained at nonlimiting levels, growth rates and ratios of dry weight to fresh weight were found to be strongly influenced by sugar concentration. Linear relationships between respiration rate and growth rate were observed, and respiration rate was found to drop to a maintenance level after sugar had been fully depleted from the medium. Diosgenin biosynthesis by D. deltoidea was shown to be independent of growth rate. Ajmalicine biosynthesis in C. roseus was negligible during sugar‐limited growth but was induced by inoculation into an 80‐g/L glucose solution.