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Growth of animal cells on microbeads. I. In situ estimation of numbers
Author(s) -
Miller S. J. O.,
Henrotte M.,
Miller A. O. A.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260281004
Subject(s) - in situ , biology , biological system , chemistry , biochemical engineering , engineering , organic chemistry
Evaluation of the number of anchorage‐dependent cells growing on the surface of microbeads kept in suspension in a bioreactor is a tedious procedure fraught with many difficulties. Using the Coulter counter as a biomass probe, this article shows that the number of cells adhering to microbeads can be determined while the cells are still attached.