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Substrate concentration control by dialysis in fermentations of Escherichia coli
Author(s) -
Thompson B. G.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280909
Subject(s) - substrate (aquarium) , dialysis , chemistry , escherichia coli , sulfate , fermentation , phosphate , chromatography , bacteria , enterobacteriaceae , biochemistry , microbiology and biotechnology , biology , organic chemistry , ecology , medicine , genetics , gene
Abstract A substrate control system based upon dialysis culture techniques has been developed. In fermentations of Escherichia coli where phosphate or sulfate concentrations were controlled, the relation between the apparent specific growth rate, μ, and the phosphate concentration followed Monod kinetics, while the relation between μ and sulfate showed a sharp optimun. The pH of fermentations of E. coli was controlled by dialysis culture techniques without the fluctuations in pH associated with control by direct addition of acid or base.