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Characteristics of lipase‐catalyzed hydrolysis of olive oil in AOT–isooctane reversed micelles
Author(s) -
Han Daeseok,
Rhee Joon Shick
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280817
Subject(s) - lipase , chemistry , candida rugosa , micelle , hydrolysis , pulmonary surfactant , triacylglycerol lipase , catalysis , glycerol , chromatography , organic chemistry , nuclear chemistry , enzyme , aqueous solution , biochemistry
Candida rugosa lipase solubilized in organic solvents in the presence of both surfactant and water could catalyze the hydrolysis of triglycerides, and kinetic analysis of the lipase‐catalyzed reaction was found to be possible in this system. Among eight organic solvents tested, isooctane was most effective for the hydrolysis of olive oil in reversed micelles. Temperature effect, pH profile, K m ,app and V max,app were determined. Among various chemical compounds, Cu 2+ , Hg 2+ , and Fe 3+ inhibited lipase severely. But the enzyme activity was restorable partially by adding histidine or glycine to the system containing these metal ions. The enzyme activity was dependent on R (molar ratio of water to surfactant) and maximum activity was obtained at R = 10.5. Upon addition of glycerol to the reversed micelles, lipase activity was affected in a different fashion depending on the R values. Stability of the lipase in reversed micelles was also dependent on R , and it was most stable at R = 5.5.