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Mechanistic analysis of complex enzyme deactivations: Influence of various parameters on series‐type inactivations
Author(s) -
Sadana Ajit,
Henley James P.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280708
Subject(s) - enzyme , chemistry , substrate (aquarium) , kinetics , series (stratigraphy) , enzyme kinetics , biophysics , stereochemistry , chromatography , active site , biochemistry , biology , physics , ecology , paleontology , quantum mechanics
A series‐type enzyme deactivation model is used to model and to quantitate some more complex enzyme deacti‐vations. The influence of temperature, pH, immobilization, chemical modifier (inhibitor or protector), substrate, and metal ion on the inactivation kinetics and on the parameter values is examined. In some cases the influence of two parameters on enzyme inactivations is presented. This provides further physical insights into enzyme inactivation and stabilization processes.