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Anaerobic fermentation of Lactobacillus bulgaricus and streptococcus thermophilus on 3% nonfat dry milk with pure and mixed culture
Author(s) -
Oner M. D.,
Erickson L. E.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280616
Subject(s) - streptococcus thermophilus , lactose , fermentation , food science , lactic acid , lactobacillus , chemistry , lactobacillus delbrueckii subsp. bulgaricus , galactose , lactobacillaceae , bacteria , biology , microbiology and biotechnology , biochemistry , genetics
Two different strains of Lactobacillus bulgaricus (ATCC‐11842 and Microlife) and two strains of Streptococcus thermophilus (ATCC‐19258 and Microlife) were grown anaerobically on 3% nonfat dry milk in pure and mixed culture using batch followed by fed batch culture. Samples were collected every 30 min. Concentrations of lactose, galactose, lactic acid, and other products present were measured using high pressure liquid chromatography. A spectrophotometric method from the literature was modified and used to determine microbial biomass concentrations. Relative cell numbers of the two organisms were measured microscopically in mixed culture. The results are presented in tabular form. S. thermophilus (Microlife) showed different growth characteristics compared to the other cultures. This culture utilized most of the galactose that was formed and produced greater amounts of lactic acid and biomass.