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Immobilization of hydrogenase in nylon gel containing electron mediator and application to an artificial photosystem
Author(s) -
Nosaka Yoshio,
Kuwabara Atsushi,
Kobayashi Takaomi,
Miyama Hajime
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280319
Subject(s) - hydrogenase , desulfovibrio vulgaris , photosystem ii , chemistry , photochemistry , mediator , photosystem i , metal , hydrogen , photosynthesis , biochemistry , organic chemistry , bacteria , biology , genetics , endocrinology
Hydrogenase of Desulfovibrio vulgaris was immobilized in nylon gel containing an electron mediator. With the immobilization, the stability for storage and repeated use increased and the optimal pH was shifted to the acidic side. Photoinduced hydrogen production was observed in an artificial photosystem consisting of the hydrogenase gel, metal porphyrin, and mercaptethanol.