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Enzymatic analyses in organic solvents
Author(s) -
Kazandjian Rafi Z.,
Dordick Jonathan S.,
Klibanov Alexander M.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280315
Subject(s) - horseradish peroxidase , toluene , chemistry , benzene , peroxidase , ether , catalysis , organic chemistry , cholesterol oxidase , oxidase test , enzyme , nuclear chemistry , chromatography
Horseradish peroxidase has been found to vigorously act as a catalyst in a number of water‐immiscible organic solvents. The rates of peroxidase‐catalyzed oxidation of p –anisidine with H 2 O 2 in toluene, benzene, ethyl and butyl acetates, and ether are in the range of 10–25% of that in water (pH 7.0) at the same reactant concentrations. Per oxidase was coupled with cholesterol oxidase (which was also found to be catalytically active in organic media), and the bienzymic system was successfully used for accurate, reliable, and reproducible determination of cholesterol in toluene.