Premium
Use of lipid solvents for viral inactivation in factor VIII concentrates
Author(s) -
Mitra G.,
Wong M.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280221
Subject(s) - virus , titer , chemistry , hepatitis a virus , virology , hepatitis c virus , chromatography , biochemistry , biology
Model virus inactivation studies with lipid solvents were carried our in antihemophilic factor concentrates. The procoagulant activity obtained was ≥80% recovery with 20% amyl acetate–0.1% deoxycholate. A concurrent reduction of four logs of virus titer was obtained for model viruses provided the viral mass contained significant amounts (≥20%) of lipid. From this preliminary study it appears that further investigations in animal models may be warranted to demonstrate the inactivation of hepatitis B virus, non‐A–non‐B virus, and AIDS virus with 20% amyl acetate–0.1% deoxycholate in antihemophilic factor concentrates.