Premium
Application of immobilized heparins for isolation of human antithrombin III
Author(s) -
Mitra G.,
Hall E.,
Mitra I.
Publication year - 1986
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260280211
Subject(s) - antithrombin , chemistry , adsorption , affinity chromatography , chromatography , human plasma , elution , selectivity , heparin , biochemistry , organic chemistry , enzyme , catalysis
Immobilized heparins were prepared by six different methods, and these were utilized for affinity purification of human antithrombin III (AT‐III). Affinity support capacities (mg AT‐III/g support) were strongly influenced by immobilized active heparin concentrations. In the temperature range 5–37°C, colder temperatures favored affinity adsorption of AT‐III as well as nonspecific interactions of all proteins. For representative human‐plasma‐derived feed solutions the selectivity for AT‐III on the affinity support was dependent on relative concentrations of non‐AT‐III proteins as well as the specific mode of adsorption and elution (batch/continuous).