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Heterogeneous denaturation of enzymes: A distributed activation energy model with nonuniform activities
Author(s) -
Agarwal Pradeep Kumar
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260271106
Subject(s) - denaturation (fissile materials) , gaussian , parametric statistics , enzyme , activation energy , thermodynamics , biological system , gaussian network model , thermal , statistical physics , chemistry , standard deviation , simple (philosophy) , experimental data , biophysics , mechanics , physics , mathematics , computational chemistry , biochemistry , biology , statistics , philosophy , epistemology , nuclear chemistry
A model is proposed for the denaturation of enzymes based on heterogeneous enzyme subpopulations with nonuniform initial activities and nonzero final activities. The more frequently used simple first‐order deactivation model is a special case of the model corresponding to the standard deviation of Gaussian distribution of activation energies approaching zero. Parametric studies made with the model are capable of simulating the reported trends in experimental data on the thermal profile and activity time behavior of native as well as modified enzymes with a minimum number of adjustable parameters. The model predictions are found to be in good agreement with data reported in the literature.