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Primary particle formation in protein precipitation
Author(s) -
Nelson C. D.,
Glatz C. E.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260271007
Subject(s) - nucleation , precipitation , particle size , particle (ecology) , fractionation , particle size distribution , chemistry , chemical physics , zeta potential , chemical engineering , primary (astronomy) , homogeneous , materials science , chromatography , thermodynamics , nanoparticle , nanotechnology , geology , organic chemistry , physics , meteorology , oceanography , astronomy , engineering
Recovery of proteins frequently involves a precipitation step in which ease of recovery of the solid protein depends on the size and density of the solid particles. The solid particles are actually aggregates of much smaller primary particles. In this work, possible influences on the aggregates of primary particle properties are postulated. The role of precipitation conditions during precipitation of soy protein with acid or CaCl 2 is determined by measurement of zeta potential and size distribution. Results are consistent with a nucleation/growth model for determination of size. There is indirect evidence that fractionation occurs at the primary particle level, although the particles are homogeneous at the aggregate level.