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Kinetic modeling of the enzymatic hydrolysis of pretreated cellulose
Author(s) -
Caminal G.,
LópezSantín J.,
Solà C.
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270903
Subject(s) - cellulose , enzymatic hydrolysis , hydrolysis , chemistry , enzyme , biochemistry , chromatography
The production of sugars by the enzymatic hydrolysis of cellulose is a two‐step process that includes conversion of the intermediate cellobiose to glucose by β‐glucosidase. The hydrolysis was followed by analyzing the two sugar products (cellobiose and glucose). The enzyme showed maximum activity at pH 4.8. Thermal deactivation was significant at temperatures above 45°C. At 50°C (optimum temperature) thermal deactivation was found to follow first‐order kinetics. Several models were tested by modeling the kinetics of the reaction. Their parameter values were determined by numerical optimization, including temperature dependence. The best fitting model was a competitive product inhibition for the two reactions in the operational range.