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Regeneration of NAD(P)H by immobilized whole cells of Clostridium butyricum under hydrogen high pressure
Author(s) -
Matsunaga Tadashi,
Matsunaga Naoki,
Nishimura Shigeo
Publication year - 1985
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260270902
Subject(s) - clostridium butyricum , nad+ kinase , regeneration (biology) , chemistry , clostridium , hydrogen , biochemistry , microbiology and biotechnology , biology , enzyme , bacteria , organic chemistry , fermentation , genetics
Immobilized whole cells of Clostridium butyricum reduced both NAD + and NADP + in the presence of hydrogen at a pressure of 100 atm. The NAD + and NADP + reduction activities were 4.45 and 4.30 U/g dry cells, respectively [U = NAD(P)H regenerated, μ mol/min]. The amount of NADH regenerated by immobilized cells increased with increasing hydrogen pressure above 10 atm. Immobilized cells (6 mg dry cells) of Cl. butyricum completely converted NAD + (6.4 μmole) to NADH for 5 h, whereas only 60% of NAD + were reduced by free cells. Immobilized cells retained 89% activity after the 5‐h reactions were repeated 4 times. L ‐Alanine was continuously produced at the rate of 12.8 μmol/min g dry cells from hydrogen, ammonium, and pyruvate with immobilized Cl. butyricum ‐alanine dehydrogenase.